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. Author manuscript; available in PMC: 2012 Sep 1.
Published in final edited form as: Virology. 2011 Jul 23;417(2):410–417. doi: 10.1016/j.virol.2011.06.026

Figure 1. Detection of FV3 DNA in adult tissues during FV3 waterborne infection.

Figure 1

(A) PCR of FV3 MCP (40 cycles) on DNA extracted from tissues from one representative frog (out of 3 individuals) for each time point of exposure (1, 3, 7, 10 and 14 days) to waterborne FV3. Es: Esophagus; In: Intestine; Ki: kidneys; Vs: Ventral skin; Ds: Dorsal skin; Bl: blood leukocytes; Rb: Red blood cells. Note that doublets in some lane are both MCP amplified products as determined by southern blot with a labeled MCP cDNA probe, whereas the low molecular signal (*) is not MCP. (B) PCR of FV3 MCP on water samples used to infect the frogs in A. Ctl: Water sample before the addition of the infected immunocompromised frog. (C) PCR of FV3 MCP on DNA extracted from tissues of one representative frog exposed to waterborne FV3 for 3 hrs. Waterborne infection was performed by infecting a sub-lethally γ-irradiated frog by ip injection of 107 PFU of FV3 and leaving this animal 12 hrs in the tank before co-housing naïve frogs for various periods of time. The arrow indicates the MCP specific signal.