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. 1999 Jan 1;13(1):76–86. doi: 10.1101/gad.13.1.76

Figure 8.

Figure 8

Electrophilic agents liberate Nrf2 from repression by Keap1. Both Nrf2 and Keap1 expression plasmids were transfected into QT6 fibroblasts. After 12 hr of culture, the cells were washed with fresh medium, and then increasing amounts of DEM (A) or catechol (D) were added to the replacement medium. The QT6 cells were cultured for another 36 hr. LUC reporter activity of cells transfected with only pRBGP2 reporter (B) or the reporter and the Nrf2 expression plasmid (C), and treated with DEM, are also shown as controls. Results of three independent experiments, each of which were carried out in duplicate, are shown with the s.e.