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. Author manuscript; available in PMC: 2012 Sep 1.
Published in final edited form as: J Magn Reson. 2011 Jul 18;212(1):229–233. doi: 10.1016/j.jmr.2011.06.010

Figure 1. (H)C(CC)(CO)NH TOCSY spectra of encapsulated 1H,13C,15N-maltose binding protein (MBP) complexed with β-cyclodextrin dissolved in liquid ethane.

Figure 1

Long range correlations of side chain carbons resolved on the adjacent (i+1) amide N-H. Not all peaks shown are centered at the indicated 15N slice. Spectra obtained at 600 MHz (1H) on a Bruker Avance III spectrometer equipped with a cryoprobe. Left Panel: 200 µM MBP in 75 mM CTAB with 450 mM hexanol at a water loading (W0) of 15. The pressure was 4,500 psi. (H)C(CC)(CO) TOCSY spectrum obtained with 64 transients per FID and 32 and 40 complex points in the 15N and 13C increment time domains respectively. A 12 ms DIPSI mixing sequence was used. Right Panel: 150 µM MBP in 75 mM CTAB with 450 mM hexanol at a W0 of 15. (H)C(CC)(CO) TOCSY spectrum obtained with 176 transients per FID and 32 and 40 complex points in the 15N and 13C increment time domains respectively. The pressure was 4,500 psi. A 20 ms DIPSI mixing sequence was used.