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. Author manuscript; available in PMC: 2012 Sep 6.
Published in final edited form as: J Proteomics. 2011 May 17;74(10):1895–1905. doi: 10.1016/j.jprot.2011.04.032

Fig. 3.

Fig. 3

Western blot of selected proteins from extracts of Lum+/+ and Lum−/− corneas with protein-fold change detected by iTRAQ mass spectrometry shown along the right margin. (A) The core proteins of the corneal SLRPs decorin (Dcn) and biglycan (Bgn) are detectable after Chondroitinase ABC digestion to remove the GAG side chains of the proteoglycans; (B) ECM collagens were detected in SDS extracted fractions of the cornea loaded in three different doses. Actin was used as a comparative loading control; (C) The following cellular proteins were detected: Aldh1a1-aldehyde dehydrogenase 1a1; Krt8-cytokeratin 8; Igfbp2–Insulin growth factor binding protein 2; Adh–alcohol dehydrogenase 1.