Skip to main content
. Author manuscript; available in PMC: 2012 Oct 1.
Published in final edited form as: Int J Biochem Cell Biol. 2011 Jul 27;43(10):1523–1531. doi: 10.1016/j.biocel.2011.07.003

Figure 7.

Figure 7

TWIST2 facilitates histone acetylation of the POSTN promoter. ChIP of fibroblasts transfected with either TWIST2, Q119X or untransfected. Anti-acetylated histone H3, anti-histone H3, and IgG (negative control) antibodies were used in probing for POSTN promoter DNA pulldown. Real time PCR of selected regions shows that cells treated with TWIST2 resulted in higher acetylation across the POSTN promoter while Q119X mutant is not associated with histone acetylation. Acetylation decreases at greater distances 5′ from the transcription start site. Primers used for the amplification of DNA are depicted in figure 5B. Asterisks represent means that are significantly different (p<0.001) from one another. Error bars represent standard error of the mean.