Table 1.
Primers
| Name | Nt | Strand | Location | Sequence |
|---|---|---|---|---|
|
SRp75 deletion constructs & PCR of endogenous and transfected SRp75
| ||||
| 75-PS | 27 | S | Start of ORF | CCGCGGGTGTACATCGGCCGCCTGAGC |
| 75RRM-1N | 27 | A
|
End of RRM1 | CTCAACAATTACTCGCTCACCACAAAG |
| 75RRM-2S | 27 | S | Start of RRM2 | AGACTTATTGTGGAGAATTTGTCAAGT |
| 75RRM-2N | 27 | A
|
End of RRM2 | TAATCTGATTTTTCTCCCATTGACTTC |
| 75RS-S | 27 | S
|
Start of RS domain | GAAGTCAATGGGAGAAAAATCAGATTA |
| 75-DN | 27 | A | End of ORF | TTAGGACCTTGAGTGGGACCTAGATCT |
|
| ||||
|
PCR of endogenous and transfected tau exon 10
| ||||
| HT7S3 | 24 | S | In tau exon 7 | CAACGCCACCAGGATTCCAGCAAA |
| HT11N | 24 | A | In tau exon 11 | ATGTTGCCTAATGAGCCACACTTG |
| SPL3-LS | 27 | S | In SPL3 vector | TCTGAGTCACCTGGACAACCTCAAAGG |
| SPL3-LN | 27 | A | In SPL3 vector | ATCTCAGTGGTATTTGTGAGCCAGGGC |
|
| ||||
|
SRp75 RNAi constructs
| ||||
| In pFIV
| ||||
| 75i-1S | 23 | S | Near start of ORF | AAAGTGTGGAGCGCTTCTTTAAG |
| 75i-1N | 23 | A | Near start of ORF | AAAACTTAAAGAAGCGCTCCACA |
| 75i-2S | 23 | S | Within RS domain | AAAGGCCGCAGCAAGAGCAAAGA |
| 75i-2N | 23 | A | Within RS domain | AAAATCTTTGCTCTTGCTGCGGC |
| 75i-3S | 23 | S | Near end of ORF | AAAGTCTAGGTCCCACTCAAGGT |
| 75i-3N | 23 | A | Near end of ORF | AAAAACCTTGAGTGGGACCTAGA |
|
| ||||
| In SM2c
| ||||
| SM75-1 | 19 | S | Within ORF | GTGATTGAATTTGTATCTT (Open Biosystems V2HS_56694) |
| SM75-2 | 19 | S | Within ORF | CAGTGGATATGGTTATAGA (Open Biosystems V2HS_56696) |
| SM75-3 | 19 | S | Within ORF | GGGAACATGCCAAGTCTGA (Open Biosystems V2HS_56698) |
Nt=Length in nucleotides. S=sense, A=antisense.