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. Author manuscript; available in PMC: 2012 Sep 15.
Published in final edited form as: Virology. 2011 Aug 2;418(1):40–48. doi: 10.1016/j.virol.2011.06.028

Fig. 2.

Fig. 2

IRF3 protein and IL-23 p19 promoter activity in RAW cells expressing polymorphic IRF3. A. Western blot of IRF3 and tubulin in RAW cells following nucleofection with pCIneo (empty vector), SJLIRF3pTARGET or B10SIRF3pTARGET. Transfected RAW264.7 cells were left uninfected or infected with 3 × 105 TMEV PFU. Nine h after infection cells were lysed and 10 ug of protein immunoblotted. Numerical values are ratios of the sum of the densitometric values of three IRF3 bands divided by the densitometric value of the tubulin band. B, C. IL-23 p19 promoter activity in 3 × 105 RAW264.7 cells incubated overnight, were co-transfected with pCIneo (empty vector), SJLIRF3pTARGET or B10SIRF3pTARGET plus p19prompGL3 and pRL-SV40 vectors. Transfected RAW264.7 cells were left uninfected or infected with 3 × 105 TMEV PFU. Promoter activity in cells was measured as firefly luciferase-dependent luminescence expressed from p19prompGL3 normalized to Renilla luciferase-dependent luminescence expressed from pRL-SV40 in 24-h cell extracts divided by ratios obtained from uninfected cells. Data are means of 5 samples each of a representative experiment ± standard error; * indicates that the mean fold increase in promoter activity in uninfected cells with SJLIRF3pTARGET (pSJL-IRF3) or B10Sirf3pTARGET (pB10S-IRF3) is significantly different than promoter activity in uninfected cells with pCIneo control. Brackets indicate significant differences promoter activity in TMEV infected cells.