Effect of SJL-IRF3 and B10.S IRF3 on relative expression of IFNβ, IL-6, ISG56, and TMEV RNA after TMEV infection. Real-time PCR of IFNβ (A), ISG56 (B), IL-6 (C), and TMEV RNA (D) in uninfected or TMEV-infected RAW264.7 cells transfected with pCIneo (control), SJLIRF3pTARGET (SJL), B10SIRF3pTARGET (B10.S) as measured after 9 h. Levels of IFNβ, IL-6, ISG56, and TMEV RNA relative to GAPDH RNA were evaluated by real-time PCR. Individual means were evaluated with the Wilcoxon-Mann-Whitney rank-sum U test. Comparisons of means in which p values < 0.05 are bracketed. *P<.05, **P<.01. Data are an average + standard error of 3 replicates per group and two different experiments.