Fig. 4.
Effect of SJL-IRF3 and B10.S IRF3 on relative expression of IFNβ, ISG56, and IL-6 after polyIC stimulation. Real-time PCR of IFNβ (A), ISG56 (B), and IL-6 (C) in RAW264.7 cells transfected with pCIneo (control), SJLIRF3pTARGET (SJL), B10SIRF3pTARGET (B10.S) as measured after 9 h. Levels of IFNβ, IL-6, and ISG56 RNA relative to GAPDH RNA were evaluated by real-time PCR. Individual means were evaluated with the Student t test. Comparisons of means in which p values < 0.05 are bracketed. Data are an average +/- standard error of 3 replicates per group from a representative experiment.