Figure 10.

The NR-box 2 peptide from GRIP1 prevents and reverses p23-mediated inhibition of the TR–DNA interaction. (A) Wild-type NR-box 2 peptide (●; KHKLHRLLQDSS) or a mutant NR-box 2 peptide (○; KHKLHRAAQDSS) was added at the indicated concentrations into a reaction containing fluorescein-TREpal oligonucleotide, TR and p23 (10 μm), and anisotropy was measured. (B) Aliquots of p23 and NR-box 2 peptide were added sequentially to a reaction mix of fluorescein-TREpal oligonucleotide and TR; anisotropy was measured after each addition.