Figure 4.

The molecular chaperone p23 differentially alters the activity of intracellular receptors. The dose response behavior of GR (A), PR (B), MR (C), ER (D), AR (E), TR (F), or RAR (G) in the parent (wild-type) and p23 disruption strain (Δp23) with (wild-type █; Δp23 ●) or without (wild-type □; Δp23 ○) plasmid expression of Sba1p. Data represent average values from three independent assays; error bars, s.e.m.. (H) Western blots from yeast extract preparations are shown; sample order is wild type, wild type with plasmid expressing Sba1p, Δp23, and Δp23 with plasmid expressing Sba1p. GR, AR, ER, TR, and Sba1p were detected by immunoblotting with polyclonal antibodies.