Fig. 2.

Cell invasion under hypoxic environments. Prostate cancer cells were seeded into transwells containing Matrigel and the inserts were incubated under hypoxia for 2, 6, and 24 h, then re-culturing under aerobic conditions for 22, 18 and 0 h, respectively. Invasion was determined by counting the number of invading cells from 8 random fields/sample (original magnification, ×100), with 3 independent samples. N=normoxia. *, p < 0.05; ***, p < 0.001 (n=24, Wilcoxon test). The global effect of hypoxia on cell invasion was analyzed by the Kruskal-Wallis test.