Fig. 2. Viral replication in WT and −/− cells.

A) i. The indicated BMK cell lines were infected with VSV (moi=0.001 or 0.0001) for 16 h, the conditioned medium was harvested and applied to indicator HT1080 cells in Log₁₀ dilutions. After an additional 16–24 h, indicator cells were fixed and stained with crystal violet and viral titers quantified as the dilution providing 50% cytopathicity, and expressed as a value relative to that observed in WT BMK infected with moi=0.001. ii. To assess viral RNA accumulation, cells were infected with VSV (moi=0.001) for 0–96 h and then total cellular RNA was isolated and reverse transcribed into cDNA that was used as a template for RT-PCR analysis of VSV G-protein mRNA expression. GAPDH served as a cellular RNA control. B) WT BMK cells were infected with VSV (moi=0.001) for 16 h and then fixed and stained for immunofluorescent detection of VSV using a G-protein primary antibody and a FITC-conjugated goat anti-mouse secondary antibody. Cellular morphology was assessed by phase contrast microscopy, and cell nuclei were detected by DAPI staining. C) Noxa −/− BMK were infected with VSV for 16h or 96 h and then fixed and stained as in “B”.