Figure 2.
Mutant Kras activation promotes increased goblet cells and Paneth cell loss, via RAS-MAPK-dependent signaling. Paneth cells, with lysozyme staining (left side, panels A and B) and goblet cells, with Alcian Blue staining (right side, panels A and B) in ileum. Scale bars, 100 μm. (C) Ratio of goblet cells vs. enterocytes in ileal crypts following CI-1040 treatment. (D and E) Recovery of Paneth cells in ileum of G22Cre KrasLSL-G12D mice following CI-1040 treatment. Scale bars, 50 μm. Arrowheads indicate lysozyme-positive Paneth cells. (F) Quantification of Paneth cells from G22Cre KrasLSL-G12D mice treated with vehicle or CI-1040. *P < .001.