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. Author manuscript; available in PMC: 2012 Aug 1.
Published in final edited form as: J Endod. 2011 Aug;37(8):1102–1108. doi: 10.1016/j.joen.2011.05.009

Figure 2.

Figure 2

Effects of calcium silicate cements on in vitro differentiation of OFMSCs.

Representative Western blot bands of control (α-MEM), wTC and wTC-αTCP extract-treated OFMSCs proteins showed immunoreativity to vascular cell adhesion marker-1 (VCAM-1), alkaline phosphatase (ALP), bone sialoprotein (BSP) and α-tubulin [protein loading control] (A). Densitometric analysis of immunoreactive bands (B) showed minimal changes in VCAM-1 and BSP levels relative to control cells but ALP level was slightly more reduced in OFMCS exposed to wTC-αTCP extracts. Real time PCR amplification of specific primers for the same markers demonstrated significant upregulation of their gene transcripts with ALP > VCAM-1 > BSP (C) [PCR data normalized to non-osteogenic controls and TATA binding protein as housekeeping gene]. There were no significant differences between the two cement types.