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. 2000 Feb 15;14(4):391–396.

Figure 4.

Figure 4

PTEN regulates PI(3)K-induced VEGF expression upstream of Akt in a HIF-1α dependent fashion. (A) U373 cells were cotransfected with either a VEGF–luciferase (top) or a HRE–luciferase (bottom) reporter in combination with either a constitutively active PI(3)K (p110*) or a constitutively active Akt (myr–Akt). Portions of the parental U373 and the p110* and myr–Akt-transfected cells were then retrovirally infected with PTEN. Thirty-six hours postinfection, the cells were treated under oxic or hypoxic conditions for 12 hr, followed by lysis and luciferase activity quantitation. (B) HIF-1α homozygous null and HIF-1α wild-type MEFs were cotransfected with VEGF–luciferase in combination with either a constitutively active PI(3)K (p110*) or a constitutively active Akt (myr–Akt). Portions of these cells were then retrovirally infected with PTEN. Thirty-six hours postinfection, the cells were treated with IGF-1 or exposed to hypoxic conditions for 12 hr followed by lysis and luciferase activity quantitation.