Map showing mosquito sampling sites along the eastern coast of the United States and results of hybrid detection by SNP markers, CxpG2T and CxpA2d, and ACE-2. Results were represented as percent of individuals that bear Cx. p. quinquefasciatus alleles. Dashed, dotted, and solid lines are the hybrid zone boundaries defined by DV/D,1 HotAce. 2,8 and microsatellite8 analyses, respectively.