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. 2011 Sep 1;85(3):434–441. doi: 10.4269/ajtmh.2011.10-0679

Figure 3.

Figure 3.

Design of CxpG2T SNP genotyping by PCR-ApaLI restriction assay. (A) Strategy of PCR amplification for G to T mutation. When T is present, the site contains an ApaLI sequence (GTGCAC). The solid black bar indicates the region corresponding to amplicon. The black triangle indicates the cutting position of restriction enzyme ApaLI. (B) Electrophoretic patterns of digested PCR products amplified by primers CxpG2TF and CxpG2TApaLI. S = 50 bp DNA size standard; M = pure Cx. p. pipiens f. molestus; P = pure Cx. p. pipiens f. pipiens; PxQ = hybrid of Cx. p. pipiens f. pipiens and Cx. p. quinquefasciatus; Q = pure Cx. p. quinquefasciatus.