Phylogenetic tree based on the Leptospira 16S ribosomal DNA gene. Sequences obtained in this study are indicated in sample numbers 334, 68, 109, 229, 75, and 82. Sequences were aligned in MEGA4 using CLUSTALW, and phylogenetic distances were calculated in MEGA4 using maximum-likelihood. Numbers at nodes are bootstrap support after 500 replicates.