Table 3.
CAN1 mutation rates in presence of msh6 NTR alleles expressed on ARS-CEN vectors
| Relevant Genotype (Strain) | CanRRate (× 10−7) | Relative to WT |
|---|---|---|
| MSH6a | 2.1 (1.6–2.7)g | 1.0 |
| msh2Δ1a | 5.4 (4.5–6.4) | 2.5 |
| msh3Δa | 4.8 (3.6–6.1) | 2.3 |
| msh2Δ1 msh3Δa | 8.9 (7.3–10.7) | 4.2 |
| msh6Δb | 19.2 (17.0–21.4) | 9.0 |
| msh2Δ1 msh6Δb | 55.6 (52.3–59.0) | 26.1 |
| msh3Δ msh6Δb | 33.0 (28.5–37.7) | 15.5 |
| msh2Δ1msh3Δ msh6Δb | 67.8 (61.9–73.8) | 31.8 |
| msh6-R232E K271E R289c | 2.8 (2.1–3.6) | 1.3 |
| msh2Δ1 msh6-R232E K271E R289Ec | 10.0 (8.8–11.4) | 4.7 |
| msh3Δ msh6-R232E K271E R289Ec | 8.1 (6.3–10.2) | 3.8 |
| msh2Δ1 msh3Δ msh6-R232E K271E R289Ec | 26.2 (22.3–30.4) | 12.3 |
| msh6Δ290–299d | 4.9 (4.0–5.9) | 2.3 |
| msh2Δ1 msh6Δ290–299d | 25.0 (22.5–27.6) | 11.7 |
| msh3Δ msh6Δ290–299d | 13.9 (11.2–16.8) | 6.5 |
| msh2Δ1 msh3Δ msh6Δ290–299d | 41.3 (36.7–46.1) | 19.4 |
| msh6Δ269–299e | 6.1 (4.9–7.3) | 2.9 |
| msh2Δ1 msh6Δ269–299e | 40.0 (36.8–43.4) | 18.8 |
| msh3Δ msh6Δ269–299e | 14.6 (12.0–17.4) | 6.9 |
| msh2Δ1 msh3Δ msh6Δ269–299e | 44.7 (38.5–51.2) | 21.0 |
| msh6Δ252–299f | 7.1 (6.0–8.2) | 3.3 |
| msh2Δ1 msh6Δ252–299f | 35.9 (32.1–39.9) | 16.9 |
| msh3Δ msh6Δ252–299f | 17.4 (14.4–20.7) | 8.2 |
| msh2Δ1 msh3Δ msh6Δ252–299f | 62.7 (54.5–71.4) | 29.5 |
a
msh6Δ strains transformed with pCK1 encoding MSH6.
b
msh6Δ strains transformed with the empty vector pRS416.
c
msh6Δ strains transformed with pSP6 encoding msh6 K232E K271E R289E.
d
msh6Δ strains transformed with pSP3 encoding msh6Δ290–299.
e
msh6Δ strains transformed with pSP5 encoding msh6Δ269–299.
f
msh6Δ strains transformed with pSP4 encoding msh6Δ252–299.
g
Canavanine assays were performed as described in Materials and Methods. Strains used are shown in Tables S1 and S2. 95% confidence intervals are in parentheses.