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. Author manuscript; available in PMC: 2012 Mar 1.
Published in final edited form as: J Immunol. 2011 Jan 26;186(5):3006–3014. doi: 10.4049/jimmunol.1003217

Figure 3. Increased responses to CpG B stimulation in IRAK2-deficient macrophages.

Figure 3

Figure 3

(A) Wild-type and IRAK2-deficient BM-derived macrophages were treated with LPS (1 μg/ml), R848 (1 μg/ml) or CpG B (1 μg/ml) for the indicated times. Cell lysates were analyzed by the Western method with antibodies against IRAK2, phospho-IKBα, IKBα, phospho-ERK, phospho-p38, and actin. (B) Wild-type or IRAK2-deficient BM-derived macrophages were treated with R848 (1 μg/ml) or CpG B (1 μg/ml) for 2 h. A heat map is shown for some genes from Illumina microarray analyses that were induced less by R848 but more by CpG in IRAK2-deficient macrophages. (C) Real-time PCR analysis of selected genes from microarray analysis. Wild-type and IRAK2-deficient BM-derived macrophages were treated with R848 or CpG B for the indicated times or were untreated. (* p< 0.05).