FIG 1 .

InMAD strategy for identifying secreted/shed bacterial antigens. BALB/c mice were infected with a pathogen, and InMAD sera were harvested when mice become moribund. Filtering the InMAD sera removed whole bacteria, and secreted/shed bacterial antigens in the sample passed through the filter. Syngeneic mice were then immunized with the InMAD sera combined with adjuvant. InMAD immune sera were harvested from the immunized mice and used to probe bacterial cell lysates by Western blotting or a bacterial proteome array.