TABLE 1 .
Analysis of serum and urine samples from melioidosis patients for CPS by Western blotting and antigen capture ELISA
| Patient sample | CFUa | Western blotb | ELISA titerc |
|---|---|---|---|
| Urined | |||
| UID1 | 2.25 × 104 | − | 8 |
| UID2 | >1 × 105 | + | 1,300 |
| UID3 | 7.5 × 104 | + | 59 |
| UID4 | 1.2 × 104 | − | 2.8 |
| UID5 | >1 × 105 | + | 200 |
| UID6 | 3.5 × 103 | − | 0 |
| UID7 | >1 × 105 | + | 550 |
| UID9 | <1 × 103 | − | 0 |
| UID10 | 1 × 103 | − | 0 |
| UID12 | 1 × 103 | − | 0 |
| Serumd | |||
| MSID1 | + | − | 16 |
| MSID2 | + | − | 0 |
| MSID3 | + | − | 20 |
| MSID4 | + | − | 9.8 |
| MSID5 | + | − | 0 |
| MSID6 | + | − | 2.5 |
| MSID7 | + | − | 0 |
| MSID8 | + | − | 0 |
| MSID9 | + | − | 4.6 |
| MSID10 | + | − | 0 |
a
Quantitative cultures were done with urine samples, and results are reported as the number of CFU/ml. Blood cultures (serum) are reported only as positive or negative. All samples were filtered before analysis for antigen.
b
Western blotting was prepared from patient urine or serum and was probed with the CPS MAb 3C5.
c
The ELISA titer is the highest dilution of patient urine or serum that produced an OD450 of ≥0.5 in an antigen capture ELISA prepared from MAb 3C5.
d
The serum and urine samples were collected from different patients.