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. 2011 Aug 1;121(9):3479–3491. doi: 10.1172/JCI43428

Figure 3. Effect of different MYST4 siRNAs on H3 and H4 acetylation.

Figure 3

(A) Results of quantitative RT-PCR revealed significantly reduced MYST4 mRNA expression levels in both cell lines (*P < 0.05, t test), HEK293 and HeLa, after exposure to 3 different MYST4 siRNAs (1, HSS118879; 2, HSS118880; 3, HSS118881). (B) Significant decrease of global H3 but (C) normal H4 acetylation was confirmed in HEK293 and HeLa cell lines (*P < 0.05, t test; **P < 0.00001, t test). Note the correlation between MYST4 depletion levels and loss of H3 acetylation (P < 0.02793, rank correlation coefficient) but not H4 acetylation (P < 0.9349, rank correlation coefficient). Based on these results the siRNA HSS118880 was used for further analysis. Numbers in the bars are the relative quantification.