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. 2011 Aug 1;121(9):3479–3491. doi: 10.1172/JCI43428

Figure 6. Biochemical characterization of MEK, ERK, and AKT phosphorylation levels.

Figure 6

(A) Increased MAPK signaling activity of the patient (P1) containing the MYST4 mutation compared with 3 control samples (C1–C3). The patient cell line transiently transfected with an MYST4 wild-type construct (R) demonstrated a normalization of the phosphorylation levels. Cells were analyzed for the phosphorylation level of MEK/pMEK1/2 (MAP2K/pMAP2K1/2), ERK/pERK1/2, and AKT/pAKT. Total amounts of MEK, ERK, and AKT and actin in cell lysates are shown, and the specificity of the antibody is specified below each panel. (B) After normalization to actin, densitometric analysis confirmed significantly increased ratios of all 3 measured parameters (*P < 0.05, t test) and normalization to wild-type ratios after rescue of MYST4 expression levels.