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The ARS5RX becomes active in a sir3 strain. 2D gels analyzing activity of ARS5RX in SIR+ strain (AR120) and sir3 (UCC4403). The arrow points to the arc characteristic of an active replication origin in UCC4403. The shapes of the arcs are somewhat atypical because the fragment containing ARS5RX is relatively large (5.2 kb) and the origin is asymmetrically located on the fragment.
