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. 2011 Aug 15;121(9):3689–3700. doi: 10.1172/JCI45709

Figure 5. PA28αOE reduces CryABR120G-induced aberrant protein aggregation.

Figure 5

(A) Western blot analyses of total ubiquitinated proteins in mouse ventricular myocardium. Representative images and pooled densitometry data (n = 4 mice/group) are shown. Total CryAB protein levels were probed to verify CryABR120G overexpression in the PA28α/tTA/CryABR120G triple-Tg mice. α-actinin was probed as a loading control. *P < 0.05 vs. non-Tg; #P < 0.05 vs. tTA/CryABR120G. (B and C) Ventricular myocardium from WT CryAB Tg, tTA/CryABR120G double-Tg, or PA28α/tTA/CryABR120G triple-Tg mice was processed for the filter trapping assay (see Methods). The proteins retained on the filter were immunoprobed for CryAB (B) or ubiquitin (C). Summarized densitometry data are also shown. *P < 0.05, **P < 0.01 vs. WT CryAB; #P < 0.05, ##P < 0.01 vs. tTA/CryABR120G.