Figure 3.

 Morphological changes induced following restoration of pRB function in SAOS2 osteogenic sarcoma cells. SAOS2 osteogenic sarcoma cells were stably transfected with plasmids encoding the indicated pRB proteins. (Left) Phase-contrast micrographs of cells following 2 weeks of selection in G418. (Right) von Kossa Staining for mineral deposition after 2 weeks selection in G418 followed by 2 weeks incubation in media containing 50 μg/ml l-ascorbic acid and 10 mm β-glycerol-phosphate. Magnification, 300×.