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. 1998 Jan 1;12(1):67–83. doi: 10.1101/gad.12.1.67

Figure 6.

Figure 6

 Dominant-negative effect of the eIF4A R362Q mutant protein on cap-mediated translation and its lack of effect on HCV and CSFV IRES-mediated translation. Reticulocyte lysate (10 μl) was preincubated alone (lanes 1,4), with eIF4A wild-type (1.2 μg) (lanes 2,5), or eIF4A mutant (1.2 μg) (lanes 3,6) for 5 min at 30°C, then incubated for 60 min at 30°C with dicistronic mRNAs (0.5 μg) as described in Materials and Methods. Translation products were analyzed by autoradiography after electrophoresis on SDS–17% polyacrylamide gel.