Figure 9.

 UV cross-linking of ribosomal protein S9 to HCV and CSFV IRESs. (A) Proteins from rabbit ribosomal 40S subunits (lane 1) or from binary complexes of rabbit 40S ribosomal subunits and [³²P]UTP-labeled HCV nucleotides 40–372 RNA (lane 2) were resolved by gel electrophoresis directly (lane 1) or after UV cross-linking and RNase digestion (lane 2). The positions of molecular mass marker proteins are indicated to the left of lane 1. (B) Proteins from binary complexes of rabbit 40S ribosomal subunits and [³²P]UTP-labeled HCV nucleotides 40–372 RNA (lane 1), HCV nucleotides 40–331 RNA (lane 2), CSFV nucleotides 1–442 RNA (lane 3), or CSFV nucleotides 1–442(Δ nucleotides 145–148) (lane 4) were resolved by gel electrophoresis after UV cross-linking, and RNase digestion. (C) Proteins from binary complexes of rabbit 40S ribosomal subunits and [³²P]UTP-labeled HCV nucleotides 40–372Δ26–67 RNA (lane 1), HCV nucleotides 40–372Δ172–227 RNA (lane 2), HCV nucleotides 40–372Δ229–238 RNA (lane 3), HCV nucleotides 40–372 (lane 4), CSFV nucleotides 1–442 (TC₃₂₅GA₃₅₇) (lane 5), CSFV nucleotides 1–442 (ΔA₃₄₉–A₃₅₃) (lane 6), CSFV nucleotides 1–442 (GA₃₅₇) (lane 7), or CSFV nucleotides 1–442 (TC₃₂₅) (lane 8) were resolved by gel electrophoresis after UV cross-linking, and RNase digestion. Proteins in A (lane 1) were visualized by staining with Coomassie blue; radio-labeled proteins in all other lanes in A,B, and C were visualized by autoradiography.