| Component | Volume 1 Reaction | Volume 5 Reactions |
| dH2O, sterile | 9 μl | 45 μl |
| 2x PCR Master Mix | 12.5 μl | 62.5 μl |
| Primer Mix | 2.5 μl | 12.5 μl |
| Total volume | 24 μl | 120 μl |
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