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Injection of 17β-estradiol (E2) into the pectoralis muscle produces elevated brain E2 content, relative to saline-injected controls (n = 3 each; *p < 0.05 for between group comparisons for each brain region). CB, cerebellum; OT, optic tectum; AH, anterior hypothalamus; HP, hippocampus; MBT, mediobasal telencephalon (containing avian amygdala); AT, anterior telencephalon; PT, posterior telencephalon.
