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. 1997 Aug 1;11(15):1974–1986. doi: 10.1101/gad.11.15.1974

Figure 3.

Figure 3

 MDM2(50–324) can inhibit basal transcription when brought to a promoter in the absence of p53, and this inhibition does not require TAFs or TFIIA. G4–MDM2(50–324) was assayed for its ability to inhibit basal transcription in a Gal4 site-dependent manner by a G-less protocol. Each reaction contains two templates that give rise to different length products: One template contains five Gal4 sites upstream of the AdMLP promoter and a G-less cassette, and one template lacks the Gal4 sites. The template giving rise to each of the two products is indicated on the left. (Lanes 1–5) The basal factors are supplied by partially purified HeLa cell nuclear fractions and recombinant hTFIIA as in Fig. 1; (lanes 6–10) purified, recombinant human TBP, TFIIB, TFIIF, TFIIE, and highly purified HeLa cell Pol II and TFIIH. Basal levels of transcription are shown in lanes 1 and 10. Lanes 2–5 and lanes 6–9 contain 7, 20, 60, and 200 ng of G4–MDM2(50–324).