Figure 2. Ligand-binding (A) and reporter assay (B) results for ESA bound to PPARγ with rosiglitazone (Ros) as a positive control.

(A) Ligand binding was assessed as a measure of mean polarization for the displaced Fluormone™ molecule versus increasing concentrations of either ligand. (B) Reporter activity was measured as relative luciferase activity for various concentrations of ESA versus 1 µM Ros. Error bars represent standard deviation, while asterisks (*) indicate significance (p≤0.05) between the data sets.