Table 1.

 α2p/Mcm1p sites and plasmids used in this study

Site	Sequence	Plasmid
		repress	activate

Uppercase letters represent the sequences of the genomic α2p/Mcm1p sites from the loci listed on the left that were cloned into the plasmids listed on the right to test for transcriptional repression (repress) and transcriptional activation (activate activity), results from which are presented in Fig. 4. Lowercase letters represent nucleotides that were added to facilitate cloning into the plasmid. Underlined sequence represents the Mcm1p-binding domain at each site; the italicized sequence represents the α2p-binding domains. The sequences of the mutant alleles of DPS1 and DPS2 are also shown, indicating the altered (bold) or deleted (space) base pairs. The mutation on the left side of dps1 created a ClaI restriction site; the mutation on the right side of dps1 created a BstBI restriction site; the mutation on the left side of dps2 created a PvuII restriction site; the mutation on the right side of dps2 created an EagI site. These restriction sites were used to verify the presence of the mutant alleles in every spore clone used in donor preference analysis.