Table 3.
Strains used in this study
| Strain
|
Genotypea
|
|---|---|
| LS255 | hmlα1α2Δ–inc/hmlα1α2Δ–inc MATa/MATαhmra1Δ101–inc/hmra1Δ101–inc HO/HO leu2-3,112/leu2-3,112 his3Δ1/his3Δ1 ura3-52/ura3-52 trp1-289/trp1-289 |
| Y2365 | LS255, MATa/MATα–inv(16–285 kb) |
| Y2366 | LS255, MATa–inv(16–285 kb)/MATα |
| Y2367 | LS255, MATa/MATα–inv(24–285 kb) |
| Y2368 | LS255, MATa–inv(24–285 kb)/MATα |
| Y2369 | LS255, MATa/MATα–inv(60–285 kb) |
| Y2370 | LS255, MATa–inv(60–285 kb)/MATα |
| Y2371 | LS255, MATa/MATα–inv(92–285 kb) |
| Y2372 | LS255, MATa–inv(92–285 kb)/MATα |
| Y2373 | LS255, MATa/MATα–inv(185–200 kb) |
| Y2374 | LS255, MATa–inv(185–200 kb)/MATα |
| Y2375 | LS255, MATa/MATα–inv(185–224 kb) |
| Y2376 | LS255, MATa–inv(185–224 kb)/MATα |
| Y2377 | LS255, MATa/MATα–inv(92–200 kb) |
| Y2378 | LS255, MATa–inv(92–200 kb)/MATα |
| Y2379 | LS255, MATa/MATα–inv(24–200 kb) |
| Y2380 | LS255, MATa–inv(24–200 kb)/MATα |
| Y2381 | LS255, MATa/MATα–inv(24–200 kb)∷pLS41 |
| Y2382 | LS255, MATa–inv(24–200 kb)∷pLS41/MATα |
| Y2383 | LS255, (dps1 dps2)Δ28961–30803/DPS1 DPS2 |
| Y2384 | LS255, dps1Δ28961–30183/DPS1 |
| Y2385 | LS255, dps2Δ29963–30803/DPS2 |
| Y2386 | LS255, dps2Δ30145–30739/DPS2 |
| Y2387 | LS255, DPS2Δ30739–30997/DPS2 |
| Y2388 | LS255, dps1Δ/DPS1 |
| Y2389 | LS255, dps2Δ/DPS2 |
| Y2390 | LS255, dps1Δ dps2Δ/DPS1 DPS2 |
| Y2391 | LS255, dps1-α2Δ/DPS1 |
| Y2392 | LS255, dps2-α2Δ/DPS2 |
| Y2393 | LS255, dps1-α2Δ dps2-α2Δ/DPS1 DPS2 |
| Y2394 | LS255, dps1Δ dps2Δ/DPS1 DPS2 [YCp–DPS1–DPS2] |
| 246.1.1 | MATα trp1 leu2 ura3 his4 |
| EG123 | MATa trp1 leu2 ura3 his4 |
| GY12 | MATa his4-917 lys2-128 leu2 ura3-52 nrp2-1 |
a
Strains Y2365–Y2382 each contain an inversion within the indicated chromosome III homolog with the indicated inversion endpoints. Chromosome III in strains Y2381 and Y2382 carries 25 kb of C. crescentus DNA inserted at the inversion breakpoint between HML and MAT. Deletion alleles in strains Y2388–Y2394 are described in the footnote to Table 2.