Figure 3.

β₂m oligomer population (0.4 mg mL⁻¹ based on monomer) before (t = −10–0 min; 10 mM ammonium acetate; pH 3.6) and during WL fibril formation (t = 0–180 min; 100 mM ammonium acetate, pH 3.6). (Solid circles) β₂m monomer; (open circles) trimer; (solid triangles) hexamer; and (shaded diamonds) 9-mer. For each oligomer, signals were normalized to a value of 1 for the most intense signal detected during the time course. The data are fitted to exponential functions (solid lines). (Thick shaded line) Thioflavin-T fluorescence signal.