Figure 9.

 mCOUP-TFI mutant embryos display reduced arborization of axonal trees. Whole-mount immunohistochemistry of wild-type (A,C) and mutant E11.5 fetuses (B,D) using 2H3 anti-neurofilament antibody. (A,B) Sagittal views at the level of posterior hindbrain and anterior somite region showing arborization of the first several spinal nerves. Note the reduced arborization in the mutant fetuses. (C,D) Higher magnification of the ophthalmic branch of the trigeminal nerve. Arrowheads point to the corresponding branching points in the wild-type and mutant embryos. Note the extensive branchings in the wild type. Also, note the less extent of arborization in the bracketed region.