Table 1. Specimens subjected to 454-FLX pyrosequencing, number of reads and ratio of endogenous and contaminant sequences obtained.
Specimen | N reads | % endogenous seqs | % human seqs |
Neandertal | 155,676 | 0.32% (N = 503) | * |
Neolithic | 168,998 | 0.66% (N = 1,117) | * |
Lynx | 361,151 | 0.11% (N = 414) | 1.4% (N = 5,078) |
Myotragus | 96,357 | 0.27% (N = 260) | 0.35% (N = 337) |
*: in the case of human samples, it is impossible to discern a priori which sequences are endogenous and which are human contaminants. However, we have mitochondrial DNA estimates of the maximum potential contamination <1% in the Neandertal sample and <5% in the Neolithic sample.