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. 2011 Sep 7;101(5):1221–1230. doi: 10.1016/j.bpj.2011.07.024

Figure 4.

Figure 4

Predicted populations and free energy profiles obtained by three-state analysis of kinetic data for the ARQ (A and C) and ARR (B and D) variants of ovPrP. In panels A and B, the elementary rate constants in Table 1 were used to compute the equilibrium population (solid lines) and maximum level of transient accumulation of the I-state on a 100-μs time (dashed lines). The free energy profiles in panels C and D were calculated using the Arrhenius relationship, assuming a preexponential factor of 1 × 106 s−1. Panel E compares the free energy profiles for three-state folding of the ARQ (solid) and ARR (dashed) variants in the absence of denaturant.