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. 2011 Aug 19;7(7):1027–1036. doi: 10.7150/ijbs.7.1027

Figure 2.

Figure 2

The biological characteristics and function of Foxp3+ Tregs subset. A. The flow cytometer profiles are representatives from the stainings for CTLA, GITR, CD45RO, CCR7, IFNγ, IL-2, IL-17, IL-10 and TGFβ in Foxp3+ Treg subset. B. The suppressive ability of CD4+ Tregs and CD8+ Tregs to naïve CD4+ T cells in vitro was measured by the T cell proliferation experiment. CSFE-labeled naïve CD4+ T cells were alone or co-cultured with CD8+ Tregs cells at ratio of 1:1 in the in complete RPMI1640 medium without IL-2 in OKT3-coated 96-well plate for 5 days and analyzed by FACS detection.