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. 2011 Aug 31;6(8):e24277. doi: 10.1371/journal.pone.0024277

Figure 3. Insulin-induced phosphorylation of NCC was impaired in SPAK knockdown mpkDCT cells.

Figure 3

A. Representative blot of phosphorylation of NCC by insulin in SPAK knockdown mpkDCT cells. mpkDCT cells were incubated with insulin for 60 min at 100 nM. Insulin-induced NCC phosphorylation was impaired in SPAK knockdown mpkDCT cells, although insulin increased phosphorylation of NCC in negative control siRNA-transfected cells. B. Densitometry analysis of phosphorylation of NCC by insulin in negative control and SPAK knockdown mpkDCT cells. Values (n = 4) are expressed as the ratio to the signals in insulin-free negative control siRNA-transfected cells.