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. 2011 Aug 31;6(8):e24277. doi: 10.1371/journal.pone.0024277

Figure 4. Insulin-induced phosphorylation of NCC was impaired in WNK4 knockdown mpkDCT cells.

Figure 4

A. Representative blot of phosphorylation of NCC by insulin in WNK4 knockdown mpkDCT cells. mpkDCT cells were incubated with insulin for 60 min at 100 nM. Insulin induced SPAK and NCC phosphorylation was impaired in WNK4 knockdown mpkDCT cells, although insulin increased phosphorylation of SPAK and NCC in negative control siRNA-transfected cells. B. Densitometry analysis of phosphorylation of SPAK and NCC by insulin in negative control and SPAK knockdown mpkDCT cells. Values (n = 4) are expressed as the ratio to the signals in insulin-free negative control siRNA-transfected cells.