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. 2011 Aug 31;6(8):e24277. doi: 10.1371/journal.pone.0024277

Figure 5. Insulin phosphorylates SPAK and NCC through PI3K in mpkDCT cells.

Figure 5

Ly294002, a PI3K inhibitor, suppressed the insulin effect on SPAK (A, B) and NCC (C, D) phosphorylation in mpkDCT cells. Cells were incubated with insulin for 60 min and Ly294002 was added 30 min before insulin incubation. (A) and (C) show representative blots of phosphorylation of SPAK and NCC, respectively. (B) and (D) are densitometry analyses of phosphorylation of SPAK and NCC, respectively. The insulin effect on phosphorylation of SPAK and NCC was absent with Ly294002. Values (n = 4) are expressed as the ratio to the signals in insulin-free samples without Ly294002. *P<0.05.