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. 2011 Aug 31;6(8):e24277. doi: 10.1371/journal.pone.0024277

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Sohara et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A: Immunoblots of phosphorylation of OSR1, SPAK and NCC in the kidney from insulin-stimulated mice on a normal diet. Phosphorylation of OSR1, SPAK and NCC was significantly increased at 90 min after insulin administration, compared to signals in the vehicle group. B: Densitometry analysis of phosphorylation of OSR1, SPAK and NCC in the kidney. In densitometry analysis, values (n = 4) are expressed as the ratio to the average of the signals in the vehicle group. *P<0.05.