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. 2011 Aug 31;6(8):e24277. doi: 10.1371/journal.pone.0024277

Figure 7. Increased phosphorylation of OSR1, SPAK and NCC in the kidney from mice on a high-salt diet.

Figure 7

A: Immunoblots of phosphorylation of OSR1, SPAK and NCC in the kidney from insulin-stimulated mice on a high-salt diet. Phosphorylation of OSR1, SPAK and NCC was significantly increased at 90 min after insulin administration, compared to signals in the vehicle group. These differences between insulin-stimulated and control mice were more abundant and significant, compared with the normal diet experiment. As well as the phosphorylation of NCC, total NCC expression in the kidney was also increased in the insulin-stimulated group. B: Densitometry analyses of phosphorylation of OSR1, SPAK and NCC in the kidney. For densitometry analysis, values (n = 4) are expressed as the ratio to the average of signals in the vehicle group. *P<0.05, **P<0.01.