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. 2011 Aug 31;6(8):e24277. doi: 10.1371/journal.pone.0024277

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Sohara et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A. Representative immunoblots of phosphorylated NCC, at 90 min after insulin administration for to wild-type and SPAK knockout mice. An insulin effect on phosphorylation of NCC was not observed in SPAK knockout mouse kidney. B. Densitometry analyses of phosphorylation of NCC in the kidney from wild-type and SPAK knockout mice. For densitometry analysis, values (n = 4) are expressed as the ratio to the average of the signals in the wild-type vehicle group. **P<0.01. n.s. not significant.