Skip to main content
. 2011 Aug 31;6(8):e24277. doi: 10.1371/journal.pone.0024277

Figure 11. WNK4 is involved in the mechanism of NCC phosphorylation by insulin.

Figure 11

A. Immunoblots of phosphorylated OSR1 and SPAK, at 90 min after insulin administration to wild-type and WNK4 hypomorphic mice. An insulin effect on phosphorylation of OSR1 was not observed in WNK4 hypomorphic mouse kidney. On the other hand, phosphorylation of SPAK was decreased significantly after insulin stimulation in WNK4 hypomorphic mouse kidney. B. Densitometry analyses of phosphorylation of OSR1, SPAK and NCC in the kidney from wild-type and WNK4 hypomorphic mice. For densitometry analysis, values (n = 4) are expressed as the ratio to the average of the signals in the wild-type vehicle group. *P<0.05, **P<0.01. n.s. not significant.