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. 2000 Mar 1;14(5):585–595.

Figure 3.

Figure 3

(A) Schematic representation of WISP-1 promoter pGL2 basic luciferase reporter constructs and deletion mutants. Putative TCF/LEF (stippled boxes) and CREB (black boxes) binding sites are indicated. Their sequences are as follows; site 1 −4882 5′-CACAAAG-3′ −4876, site 2 −4677 5′-CTCAAAG-3′ −4671, site 3 −2978 5′-CTTTGAT-3′ −2972, site 4 −2546 5′-CTTTGTT-3′ −2540, site 5 −461 5′-CTCAAAG-3′ −455, CREB site −155 5′-TGACGTCA-3′ −148. (B) Response of WISP-1 promoter deletion contructs to β-catenin. The 293 cells were transfected with 0.5 μg of WISP-1 promoter, different deletion constructs, or empty pGL2 basic luciferase reporters, together with 1 μg of empty pCS2/MT vector, wild type, or 4145 β-catenin. A total of 0.1 μg of CMV–β-gal was cotransfected to normalize transfection efficiency. (C) Schematic representation of Frag3 and TCF/LEF site mutation reporter constructs. Mutated sites are as indicated. Transfections were carried out as described in B. (D) Schematic representation of Frag3, multiple TCF/LEF site mutations, or CREB site mutation. Transfections were carried out as described in B.