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. 2000 Mar 1;14(5):585–595.

Figure 5.

Figure 5

(A) Effects of dominant-negative CREB on the WISP-1 promoter reporter. The 293 cells were transfected with 0.5 μg of pCS2/MT, wild type, 4145, or 4145TV β-catenin, and 1.0 μg of pcDNA3 (left) or KCREB (right), together with 0.5 μg of WISP-1 promoter pGL2 basic luciferase reporter. A total of 0.1 μg of CMV–β-gal was cotransfected to normalize transfection efficiency. (B) Effects of dominant-negative CREB on the TopFlash reporter. A total of 1.0 μg of pcDNA3 (left) or KCREB (right) was used. Transfections were carried out as described in A except that 0.5 μg of TopFlash reporter was used.